Training

The role of flow cytometry in marine biodiversity and ecosystem functioning

The main aim of the course was to introduce graduate students and young researchers to the application of flow cytometry to marine biodiversity and ecosystem functioning. Flow cytometry itself is a very powerful generic technique which allows marine scientists to obtain information about particles in sea water, from plankton community information to cellular process information. This ability enables marine scientists to study a wide range of research topics related to marine biodiversity and ecosystem function. Specifically, the aims of the course were to:

• present a series of lectures and practical sessions by experts in the field to inform students of the state of the art of flow cytometry and its use with respect to marine biodiversity and ecosystem functioning.
• expose students to the latest flow cytometry technology through the participation of leading flow cytometer manufacturers.
• enable students to present their current research in poster papers as an aid to stimulating discussion.
• enable students to discuss topics of interest with experts and manufacturers as an aid to formulating new lines of research and helping with current research. To ensure this, all lecturers and manufacturers were present for the whole course.
• provide a platform for the creation of a new network of young researchers involved in the use of flow cytometry to study marine biodiversity and ecosystem function.
• disseminate information from the course as outlined below.

Material

• FCM poster
• Final report
• Programme
• Lectures
• Participants
• Posters
• Pictures (zip file - 13MB)
• Pictures on the MarBEF photogallery

Lectures

• Ianora, A. Introduction to MarBEF
• Dubelaar, G. Basics of flow cytometry
• Dubelaar, G. Diversity of flow cytometry and what they do
• Casotti, R. Conventional flow cytometry capabilities
• Sieracki, M. Novel/Dedicated Instruments and Technologies
• Gregori, G. Flow cytometry data handling Flow cytometry data handling and analysis and analysis
• Gasol, J. Bacteria and flow cytometry: From enumeration to community structure and ecological function
• Biegala, I.C. Probing phytoplankton biodiversity
• Li, W. patterns in the sea : indicators of change Plankton abundance patterns in the sea : indicators of change
• Veldhuis, M.J.W. Cell-related processes
• Tarran, G. Population-related processes
• Brunet, C. Ecophysiological approaches for understanding ecosystem functioning: integrating flow cytometry and environmental data

• Gasol, J. How to count picoalgae and bacteria with the FACScalibur flow cytometer - manual
• Gasol, J. Cell Sorting - protocols
• Biegala, I. TSA-FISH and flow cytometry to probe photosynthetic nano and picoeukaryotes - practical outline

Posters

• Alonso Santos. Flow cytometer applications at IMEDEA
• Felipe Alvarez. Flow cytometric measurements of microbial abundance, size and morphology, viability, and activity during a microcosm experiment
• Amalfitano. Structure-function analysis of bacterial community in acquatic systems
• Aquino Cruz. Distribution of picophytoplankton in the southern Gulf of Mexico
• Assimakopoulou,G.; M. Reckermann, K. Pagou, E. Krassakopoulou, F. Colijn. Dynamics of photosynthetic picoplankton in North Aegean Sea (Eastern Mediterranean) using flow cytometric analysis
• Azzaro. Comparison between different methods (epifluorescence microscopy, lps and flow cytometry) for the determination of the bacterioplankton biomass
• Bernardi Aubry. Picophytoplankton contribution to phytoplankton community structure in the NW Adriatic Sea (Gulf of Venice)
• Buric. Temporal and spatial distribution of phytoplankton in highly stratified estuary
• Calic. Phytoplankton in Two Small Marine Lakes (Mala Solina and Velika Solina, Middle Adriatic)
• Calvo Diaz. Seasonal dynamics of the picoplankton in the central Cantabrian Sea (S Bay of Biscay)
• Dimier. Kinetics of photoacclimation in diatoms: interspecific variability.
• Eiler. Composition, dynamics and ecophysiology of freshwater bacterial communities associated with cyanobacterial blooms
• Falcioni
• Manti. Viability of environmental Aeromonas strains in different types of water evaluated by flow cytometry and comparison with classical methods
• Garcias Bonet. Flow Cytometry applied to the study of Posidonia oceanica meristematic activity
• Gutseit K, Daniel C, Anesio A & Granéli W. Dark Limnology - Zooplankton production based on aged versus recently produced organic matter.
• Hendriks. Particle trapping in seagrass meadows
• Holm. Marine stem cell biology: The role of blood cells and stem cells for the blood in regeneration of new tissue in the marine invertebrates Ciona intestinalis and Asterias rubens
• Hubas C, Davoult D, Artigas LF, Cariou T. Assigning intertidal production and respiration to benthic organisms during emersion with an in situ method : response of microbes to seasonal changes in coarse and muddy sediments and effects of natural eutrophication caused by macroalgal deposits
• Kaartokallio. Physiological and community structure responses of sea-ice and open water bacterial communities to salinity change
• Kerros. Generic presentation of flow cytometric analysis in the Bay of Villefranche (descriptional title)
• Meier. Ploidy level determination of different life-cycle stages in calcareous dinoflagellates
• Nardi. Effect of Rare Earth Elements on Seed Germination and Antioxidant Metabolism in Triticum Durum DESF.
• Nardi. Effect of Rare Earth Application on the Growth of TrichodermaSspp. and of Several Plant Pathogenic Fungi
• Nielsen, S; Bishop, J; Hawkins, S. The effects of suspension-feeder biodiversity on ecosystem functioning and the effect of non-native suspension-feeder species.
• Parada. Diel dynamics in virioplankton
• Pitta. The response of the microbial loop during a large scale Lagrangian phosphate addition experiment in the Eastern Mediterranean by P. Pitta, N. Stambler, T. Tanaka, T. Zohary, A. Tselepides & F. Rassoulzadegan
• Psarra. Phytoplankton dynamics within oligotrophy gradients in the E. Mediterranean
• Ribalet. Effects of two diatom-derived aldehydes on teh growth of 6 marine phytoplankton species
• Santarpia, I; Margiotta, F; Saggiomo, V. Primary production processes during Lagrangian Experiments in the Northwestern Mediterranean Sea
• Segvic. Vertical distribution of picoplankton in Rogoznica Lake
• Sintes. Use of probes for analyzing activity and viability in bacterioplankton
• Smolarz. Abnormalities related to neoplasia in the Baltic clam Macoma balthica from the Gulf of Gdansk (Baltic Sea)
• Stuhrman. Microbial Diversity in the namibian upwelling region near Walvis Bay.
• Veneri. Distribution of the chlorophyll a and pigments accessories: relationship with phytoplankton populations and hydrology features in the Northern Adriatic Sea

Group picture

To look up the names, click on photo outline (pdf).

Organising institutes

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