Marine Biodiversity and Ecosystem Functioning
EU Network of Excellence

 
Main Menu

· Home
· Contacts
· Data Systems
· Documents
· FAQ
· Links
· MarBEF Open Archive
· Network Description
· Outreach
· Photo Gallery
· Quality Assurance
· Register of Resources
· Research Projects
· Rules and Guidelines
· Training
· Wiki
· Worldconference

 

Register of Resources (RoR)

 People  |  Datasets  |  Literature  |  Institutes  |  Projects 

[ report an error in this record ]basket (0): add | show Print this page

Assay optimisation and age-related baseline variation in biochemical markers in Lesser Black-backed gulls
Santos, C.S.A.; Loureiro, S.; Sotillo, A.; Müller, W.; Stienen, E.W.M.; de Neve, L.; Lens, L.; Monteiro, M.S. (2019). Assay optimisation and age-related baseline variation in biochemical markers in Lesser Black-backed gulls. Ecotoxicol. Environ. Saf. 172: 246-254. https://dx.doi.org/10.1016/j.ecoenv.2019.01.084
In: Ecotoxicology and Environmental Safety. Academic Press/Elsevier: Amsterdam, Netherlands etc. ISSN 0147-6513; e-ISSN 1090-2414
Peer reviewed article  

Available in  Authors 

Keyword
    Larus fuscus Linnaeus, 1758 [WoRMS]
Author keywords
    Biomarkers; Blood; Cholinesterase; Lactate dehydrogenase; Oxidative stress; Gulls

Authors  Top 
  • Santos, C.S.A.
  • Loureiro, S.
  • Sotillo, A.
  • Müller, W.
  • Stienen, E.W.M., more
  • de Neve, L.
  • Lens, L.
  • Monteiro, M.S.

Abstract
    Free-ranging animals are often used as bioindicators of both short- and long-term changes in ecosystem health, mainly to detect the presence and effects of contaminants. Birds, and gulls in particular, have been used as bioindicators over a broad range of marine and terrestrial ecosystems. In this study, we standardise the conditions for the use of a suite of biochemical markers in non-destructive matrices of Lesser Black-backed Gull (Larus fuscus) to facilitate future biomonitoring of marine and terrestrial contaminants. We characterized cholinesterase (ChE) in plasma and optimized assay conditions for ChE activity as a marker of neurotoxic damage. Moreover, we quantified variation in activity of ChE, lactate dehydrogenase (LDH), glutathione-S-transferase (GST) and catalase (CAT) as well as variation ranges of lipid peroxidation (LPO), in free-ranging adults and captive chicks. The main ChE form present in plasma of both adults and chicks was butyrylcholinesterase (BChE) followed by acetylcholinesterase (AChE), whose relative proportion in plasma tended to decrease with increased chick age. LPO levels and GST activity in blood cells (BCs) decreased significantly with increasing chick age, while BChE and LDH activity in plasma were not age-dependent. CAT in BCs tended to decline non-significantly in older chicks. Results of this study underscore the importance of standardising assay conditions and assessing intrinsic baseline variation in biochemical markers, before biochemical quantification. Data presented here provide a foundation for future use of BChE and LDH activity in plasma, as well as oxidative stress markers (LPO, CAT and GST) in BCs, to monitor environmental stress effects in Lesser Black-backed gulls.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Authors 


If any information here appears to be incorrect, please contact us
Back to Register of Resources
 
Quick links

MarBEF WIKI

Erasmus Mundus Master of Science in Marine Biodiversity and Conservation (EMBC)
Outreach

Science
Responsive Mode Programme (RMP) - Marie Nordstrom, copyright Aspden Rebecca

WoRMS
part of WoRMS logo

ERMS 2.0
Epinephelus marginatus Picture: JG Harmelin

EurOBIS

Geographic System

Datasets

 


Web site hosted and maintained by Flanders Marine Institute (VLIZ) - Contact data-at-marbef.org