Marine Biodiversity and Ecosystem Functioning
EU Network of Excellence

 
Main Menu

· Home
· Contacts
· Data Systems
· Documents
· FAQ
· Links
· MarBEF Open Archive
· Network Description
· Outreach
· Photo Gallery
· Quality Assurance
· Register of Resources
· Research Projects
· Rules and Guidelines
· Training
· Wiki
· Worldconference

 

Register of Resources (RoR)

 People  |  Datasets  |  Literature  |  Institutes  |  Projects 

[ report an error in this record ]basket (1): add | show Print this page

one publication added to basket [333470]
An "-omics" approach for the study of retinoic acid gene regulatory network in S. purpuratus
Burgoa Cardas, J. (2020). An "-omics" approach for the study of retinoic acid gene regulatory network in S. purpuratus. MSc Thesis. Ghent University/Stazione Zoologica Anton Dohrn (SZN): Ghent/Napoli. 47 pp.

Available in  Author 
Document type: Dissertation

Keywords
    Strongylocentrotus purpuratus (Stimpson, 1857) [WoRMS]
    Marine/Coastal

Author  Top 
  • Burgoa Cardas, J.

Abstract
    The signalling pathway of the vitamin A derivative all-trans retinoic acid (RA) has been widely studied in the past, and its relevance for normal embryonic development in a great conspicuous number of Deuterostome species is well known. Despite the thorough research on the gene regulatory networks that conform the early embryology of the model Echinoderm species Strongylocentrotus purpuratus, there is a gap of knowledge on the genetic machinery that controls RA action in this species. By means of chromatin accessibility (ATAC-seq) and gene expression analysis (RNA-seq) we describe the first genome wide in silico analysis of putative cis-regulatory modules within the RA pathway of S. purpuratus early embryonic stages. The RA hydroxylase enzyme coding gene Sp-Cyp26L showed promising potential for retinoic acid mediated regulation. The appliance of newly created bioinformatical pipelines with chromatin accessibility analysis (ATAC-seq) failed to increase the confidence on the putative cis-regulatory modules found and issues causing this outcome were revised. Additionally, new DNA motifs where the basic transcription factors of the RA machinery bind were searched, and 15 new putative motifs were found. Overall, the present work provides valuable knowledge base for the further analysis of the RA gene regulatory network of S. purpuratus.

All data in the Integrated Marine Information System (IMIS) is subject to the VLIZ privacy policy Top | Author 


If any information here appears to be incorrect, please contact us
Back to Register of Resources
 
Quick links

MarBEF WIKI

Erasmus Mundus Master of Science in Marine Biodiversity and Conservation (EMBC)
Outreach

Science
Responsive Mode Programme (RMP) - Marie Nordstrom, copyright Aspden Rebecca

WoRMS
part of WoRMS logo

ERMS 2.0
Epinephelus marginatus Picture: JG Harmelin

EurOBIS

Geographic System

Datasets

 


Web site hosted and maintained by Flanders Marine Institute (VLIZ) - Contact data-at-marbef.org